The ALDH1A2 Monoclonal Antibody (CAB9123) is a high-quality antibody developed for reliable detection and analysis of target proteins. This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene. RRID AB_2863659 Gene ID 8854 Swiss Prot Synonym DIH4; RALDH2; RALDH2-T; RALDH(II); ALDH1A2
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ALDH1A2 Monoclonal Antibody
SKU:
CAB9123
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1797
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DIH4, RALDH2, RALDH2-T, RALDH(II), ALDH1A2
Positive Sample:
K-562, Mouse testis
Cellular Localization:
Cytoplasm.
Calculated MW:
57kDa
Observed MW:
57kDa
This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene. RRID AB_2863659 Gene ID 8854 Swiss Prot Synonym DIH4; RALDH2; RALDH2-T; RALDH(II); ALDH1A2
Purification Method:
Affinity purification
Gene ID:
8854
RRID:
AB_2863659
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ALDH1A2 Rabbit mAb (CAB9123) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using ALDH1A2 Rabbit mAb (CAB9123) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
