The Cytokeratin 4 (KRT4) Monoclonal Antibody (CAB0013) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. RRID AB_2861445 Gene ID 3851 Swiss Prot Synonym K4; CK4; CK-4; CYK4; WSN1; Cytokeratin 4 (KRT4)
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Cytokeratin 4 (KRT4) Monoclonal Antibody
SKU:
CAB0013
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC1804
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAIF-P
Recommended Dilution:
WB
1:1000 - 1:4000
IF-P
1:100 - 1:400
IHC-P
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. RRID AB_2861445 Gene ID 3851 Swiss Prot Synonym K4; CK4; CK-4; CYK4; WSN1; Cytokeratin 4 (KRT4)
Purification Method:
Affinity purification
Gene ID:
3851
RRID:
AB_2861445
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cytokeratin 4 (KRT4) (KRT4) Rabbit mAb (CAB0013) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human esophagus tissue using Cytokeratin 4 (KRT4) Rabbit mAb (CAB0013) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded human breast tissue using Cytokeratin 4 (KRT4) Rabbit mAb (CAB0013) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Confocal imaging of human tonsil tissue using Cytokeratin 4 (KRT4) Rabbit mAb (CAB0013,dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer (pH 6.0) before commencing with IF staining protocol.
