The Cytokeratin 6 (KRT6) Monoclonal Antibody (CAB19827) is a high-quality antibody developed for reliable detection and analysis of target proteins. Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. RRID Gene ID 286887 Swiss Prot Synonym K6E; KRT6E; PPKNEFD; Cytokeratin 6 (KRT6)
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Cytokeratin 6 (KRT6) Monoclonal Antibody
SKU:
CAB19827
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC2352
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
K6E, KRT6E, PPKNEFD, Cytokeratin 6 (KRT6)
Positive Sample:
5637, A-431
Cellular Localization:
Cytosol, Extracellular Exosome.
Calculated MW:
60kDa
Observed MW:
60kDa
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. RRID Gene ID 286887 Swiss Prot Synonym K6E; KRT6E; PPKNEFD; Cytokeratin 6 (KRT6)
Purification Method:
Affinity purification
Gene ID:
286887
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cytokeratin 6 (KRT6) Rabbit mAb (CAB19827) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Cytokeratin 6 (KRT6) Rabbit mAb (CAB19827) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Cytokeratin 6 (KRT6) Rabbit mAb (CAB19827) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
