The ELOVL6 Polyclonal Antibody (CAB21094) is a high-quality antibody developed for reliable detection and analysis of target proteins. Fatty acid elongases (EC 6.2.1.3), such as ELOVL6, use malonyl-CoA as a 2-carbon donor in the first and rate-limiting step of fatty acid elongation (Moon et al., 2001 [PubMed 11567032]). RRID Gene ID 79071 Swiss Prot Synonym FAE; LCE; FACE; hELO2; ELOVL6
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ELOVL6 Polyclonal Antibody
SKU:
CAB21094
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FAE, LCE, FACE, hELO2, ELOVL6
Positive Sample:
U-251MG, HepG2, SK-BR-3, Mouse liver, Rat liver, Mouse kidney, Rat liver, Mouse kidney
Cellular Localization:
Endoplasmic Reticulum.
Calculated MW:
31kDa
Observed MW:
31kDa
Fatty acid elongases (EC 6.2.1.3), such as ELOVL6, use malonyl-CoA as a 2-carbon donor in the first and rate-limiting step of fatty acid elongation (Moon et al., 2001 [PubMed 11567032]). RRID Gene ID 79071 Swiss Prot Synonym FAE; LCE; FACE; hELO2; ELOVL6
Purification Method:
Affinity purification
Gene ID:
79071
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse kidney using ELOVL6 Rabbit pAb (CAB21094) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time:30s.
Western blot analysis of lysates from Rat liver using ELOVL6 Rabbit pAb (CAB21094) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) Exposure time: 10 s.
