The Syntaxin 4 Monoclonal Antibody (CAB5996) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables sphingomyelin phosphodiesterase activator activity. Involved in several processes, including cornified envelope assembly; positive regulation of immune effector process; and positive regulation of protein localization. Located in several cellular components, including basolateral plasma membrane; cytoplasmic vesicle; and lamellipodium. Part of SNARE complex. Is active in glutamatergic synapse and postsynapse.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Syntaxin 4 Monoclonal Antibody
SKU:
CAB5996
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2113
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:10000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
STX4A, p35-2, Syntaxin 4
Positive Sample:
A549, Mouse lung, Rat brain
Cellular Localization:
Basolateral Plasma Membrane, Cytosol, Dendritic Spine, Endosome, Extracellular Exosome, Extracellular Space, Glutamatergic Synapse, Lateral Loop, Myelin Sheath Adaxonal Region, Neuron Projection Membrane, Perinuclear Region Of Cytoplasm, Plasma Membrane, Postsynapse, Presynaptic Active Zone Membrane, Snare Complex, Synapse, Synaptic Vesicle, Trans-Golgi Network.
Calculated MW:
34 kDa
Observed MW:
34 kDa
Enables sphingomyelin phosphodiesterase activator activity. Involved in several processes, including cornified envelope assembly; positive regulation of immune effector process; and positive regulation of protein localization. Located in several cellular components, including basolateral plasma membrane; cytoplasmic vesicle; and lamellipodium. Part of SNARE complex. Is active in glutamatergic synapse and postsynapse.
Purification Method
Affinity purification
Gene ID
6810
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Syntaxin 4 Rabbit mAb (CAB5996) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Rat brain, using Syntaxin 4 Rabbit mAb (CAB5996) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
