The Aquaporin-4 (AQP4) Monoclonal Antibody (CAB11210) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Additional isoforms, resulting from the use of alternative in-frame translation initiation codons, have also been described. Recent studies provided evidence for translational readthrough in this gene, and expression of C-terminally extended isoforms via the use of an alternative in-frame translation termination codon.
This antibody is validated for use in WB, IHC-P, ELISA, IF-F, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Aquaporin-4 (AQP4) Monoclonal Antibody
SKU:
CAB11210
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC54345
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAIF-FIF-P
Recommended Dilution:
WB
1:5000 - 1:20000
IF-F
1:200 - 1:1000
IF-P
1:200 - 1:800
IHC-P
1:200 - 1:800
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.For high-ratio antibody dilutions (≥1:10000)，a sequential dilution method is strongly recommended to ensure measurement accuracy.
Synonyms:
MIWC, WCH4, hAQP4, Aquaporin-4 (AQP4)
Positive Sample:
Rat brain, Mouse brain
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
32KDa/34kDa
Observed MW:
28 kDa
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Additional isoforms, resulting from the use of alternative in-frame translation initiation codons, have also been described. Recent studies provided evidence for translational readthrough in this gene, and expression of C-terminally extended isoforms via the use of an alternative in-frame translation termination codon.
Purification Method
Affinity purification
Gene ID
361
RRID
AB_2861521
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Aquaporin-4 (AQP4) Rabbit mAb (CAB11210) at 1:4000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): Rat liver. Exposure time: 20 s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Aquaporin-4 (AQP4) Rabbit mAb (CAB11210) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Aquaporin-4 (AQP4) Rabbit mAb (CAB11210) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Aquaporin-4 (AQP4) Rabbit mAb (CAB11210) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Aquaporin-4 (AQP4) Rabbit mAb (CAB11210) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Aquaporin-4 (AQP4) Rabbit mAb (CAB11210) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Aquaporin-4 (AQP4) Rabbit mAb (CAB11210) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of paraffin-embedded mouse brain tissue using Aquaporin-4 (AQP4) Rabbit mAb (CAB11210, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded rat brain tissue using Aquaporin-4 (AQP4) Rabbit mAb (CAB11210, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
