ATP6V0D2 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ul format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
ATP6V0D2 Antibody (PACO62527)
SKU:
PACO62527
Size:
50μl
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant Human V-type proton ATPase subunit d 2 protein (50-295AA)
Immunogen Species:
Homo sapiens (Human)
Uniprot No:
Q8N8Y2
Form:
Liquid
Tested Applications:
ELISAWBIHCIF
Recommended Dilution:
WB 1:1000-1:5000, IHC 1:500-1:1000, IF 1:50-1:200
Synonyms:
ATP6V0D2V-type proton ATPase subunit d 2 antibody, V-ATPase subunit d 2 antibody, Vacuolar proton pump subunit d 2 antibody
Western Blot Positive WB detected in: A549 whole cell lysate, PC3 whole cell lysate All lanes: ATP6V0D2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa
IHC image of PACO62527 diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO62527 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
