The Carbonic Anhydrase 1 (CA2) Monoclonal Antibody (CAB9148) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. RRID AB_2863671 Gene ID 760 Swiss Prot Synonym CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282; Carbonic Anhydrase 2 (CA2)
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Carbonic Anhydrase 1 (CA2) Monoclonal Antibody
SKU:
CAB9148
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1451
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
293T, Mouse spinal cord, Mouse brain, Mouse kidney, Rat spinal cord, Rat brain
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
29kDa
Observed MW:
29kDa
The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. RRID AB_2863671 Gene ID 760 Swiss Prot Synonym CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282; Carbonic Anhydrase 2 (CA2)
Purification Method:
Affinity purification
Gene ID:
760
RRID:
AB_2863671
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Carbonic Anhydrase 2 (CA2) Rabbit mAb (CAB9148) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Carbonic Anhydrase 2 (CA2) Rabbit mAb (CAB9148) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Carbonic Anhydrase 2 (CA2) Rabbit mAb (CAB9148) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Carbonic Anhydrase 2 (CA2) Rabbit mAb (CAB9148) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Carbonic Anhydrase 2 (CA2) Rabbit mAb (CAB9148) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
