The B7-H3/CD276 Antibody (CAB17216) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2768778 Gene ID 80381 Swiss Prot Synonym B7H3; B7-H3; B7RP-2; 4Ig-B7-H3; CD276/B7-H3
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
B7-H3/CD276 Antibody
SKU:
CAB17216
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
-
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
B7H3, B7-H3, B7RP-2, 4Ig-B7-H3, CD276/B7-H3
Positive Sample:
U-2 OS, LNCaP
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
57kDa
Observed MW:
90kDa
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2768778 Gene ID 80381 Swiss Prot Synonym B7H3; B7-H3; B7RP-2; 4Ig-B7-H3; CD276/B7-H3
Purification Method:
Affinity purification
Gene ID:
80381
RRID:
AB_2768778
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CD276/B7-H3 Rabbit pAb (CAB17216) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): Raji Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using CD276/B7-H3 Rabbit pAb (CAB17216) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
