The CDK9 Antibody (CAB0886) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. RRID AB_2757443 Gene ID 1025 Swiss Prot Synonym TAK; C-2k; CTK1; CDC2L4; PITALRE; CDK9
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CDK9 Antibody
SKU:
CAB0886
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TAK, C-2k, CTK1, CDC2L4, PITALRE, CDK9
Positive Sample:
HeLa, 293T, MCF7, Rat testis
Cellular Localization:
Cytoplasm, Nucleus, Pml Body.
Calculated MW:
43kDa
Observed MW:
40kDa
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. RRID AB_2757443 Gene ID 1025 Swiss Prot Synonym TAK; C-2k; CTK1; CDC2L4; PITALRE; CDK9
Purification Method:
Affinity purification
Gene ID:
1025
RRID:
AB_2757443
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CDK9 Rabbit pAb (CAB0886) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using CDK9 Rabbit pAb (CAB0886) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver using CDK9 Rabbit pAb (CAB0886) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
