The CHMP4C Antibody (PAC04694) is a high-quality polyclonal antibody designed for research involving CHMP4C, a protein involved in the endosomal sorting complex required for transport (ESCRT) pathway. This antibody is raised in rabbits and has been validated for use in various applications, including Western blot and immunofluorescence.CHMP4C is a key player in cellular processes such as multivesicular body biogenesis, cytokinesis, and viral budding, making it essential for studying intracellular trafficking and membrane dynamics. This antibody specifically targets the CHMP4C protein, allowing for precise detection and analysis in a variety of experimental settings.
Researchers in cell biology, virology, and cancer research will find this antibody particularly useful for investigating the role of CHMP4C in cellular function and disease processes. By utilizing this antibody, scientists can gain valuable insights into the mechanisms underlying vesicle formation, membrane remodeling, and viral replication, ultimately advancing our understanding of fundamental biological processes.
Antibody Name:
CHMP4C Antibody (PACO46994)
Antibody SKU:
PACO46994
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Charged multivesicular body protein 4c protein (1-233AA)
Immunohistochemistry of paraffin-embedded human colon cancer using PACO46994 at dilution of 1:100.
Background:
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: upon phosphorylation by AURKB, together with ZFYVE19/ANCHR, retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ANCHR and VPS4 and subsequent abscission. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan.
Synonyms:
Charged multivesicular body protein 4c (Chromatin-modifying protein 4c) (CHMP4c) (SNF7 homolog associated with Alix 3) (SNF7-3) (hSnf7-3) (Vacuolar protein sorting-associated protein 32-3) (Vps32-3) (hVps32-3), CHMP4C, SHAX3
UniProt Protein Function:
CHMP4C: component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex, it probably serves as an acceptor for ESCRT-I complex on endosomal membranes. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins, via the association of CHMP4 proteins with PDCD6IP/AIP1, a protein directly recruited by HIV-1 p6 protein that functions at sites of viral Gag assembly and budding.Protein type: VesicleChromosomal Location of Human Ortholog: 8q21.13Cellular Component: late endosome membrane; midbody; cytosolMolecular Function: protein binding; protein homodimerization activityBiological Process: protein transport; cell separation during cytokinesis; viral reproduction; abscission; vacuolar transport; regulation of viral reproduction; viral infectious cycle; endosome transport; mitotic metaphase plate congression; negative regulation of cytokinesis; nuclear organization and biogenesis
UniProt Protein Details:
NCBI Summary:
CHMP4C belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]
Chromatin-modifying protein 4c; CHMP4c; SNF7 homolog associated with Alix 3; SNF7-3; hSnf7-3; Vacuolar protein sorting-associated protein 32-3; Vps32-3; hVps32-3
