The CLCN1 Monoclonal Antibody (CAB21237) is a high-quality antibody developed for reliable detection and analysis of target proteins. The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. The protein encoded by this gene regulates the electric excitability of the skeletal muscle membrane. Mutations in this gene cause two forms of inherited human muscle disorders: recessive generalized myotonia congenita (Becker) and dominant myotonia (Thomsen). Alternative splicing results in multiple transcript variants. RRID Gene ID 1180 Swiss Prot Synonym CLC1; CLCN1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CLCN1 Monoclonal Antibody
SKU:
CAB21237
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC52491
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CLC1, CLCN1
Positive Sample:
293T transfected with CLCN1
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
109kDa
Observed MW:
109 kDa
The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. The protein encoded by this gene regulates the electric excitability of the skeletal muscle membrane. Mutations in this gene cause two forms of inherited human muscle disorders: recessive generalized myotonia congenita (Becker) and dominant myotonia (Thomsen). Alternative splicing results in multiple transcript variants. RRID Gene ID 1180 Swiss Prot Synonym CLC1; CLCN1
Purification Method:
Affinity purification
Gene ID:
1180
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CLCN1 (Human) using CLCN1 Rabbit mAb (CAB21237) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 20 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) .Exposure time: 45 s.
