The E2F8 Antibody (CAB1135) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. RRID AB_2758519 Gene ID 79733 Swiss Prot Synonym E2F-8; E2F8
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
E2F8 Antibody
SKU:
CAB1135
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
E2F-8, E2F8
Positive Sample:
SW480, Mouse testis
Cellular Localization:
Nucleus.
Calculated MW:
94kDa
Observed MW:
105kDa
This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. RRID AB_2758519 Gene ID 79733 Swiss Prot Synonym E2F-8; E2F8
Purification Method:
Affinity purification
Gene ID:
79733
RRID:
AB_2758519
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using E2F8 Rabbit pAb (CAB1135) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020).
