FDG Beta-Galactosidase Assay Kit is an efficient, easy and highly sensitive tool to quickly measure the levels of active Beta-galactosidase expressed in cells transfected with plasmids expressing Lac Z.
Lac Z is often used reporter gene in experiments transfection because the Beta-galactosidase is very resistant to proteolytic degradation and its activity is easily measured.
Beta-galactosidase performs the hydrolysis of fluorescein di-Beta-D galactopyranoside (FDG) into a fluorescein that can be detected at excitation/emission = 490/525 nm. The concentration of Beta-galactosidase is proportional to fluorescence produced.
Measure levels of active Beta-galactosidase expressed in cells transfected with plasmids expressing Lac Z.
