The FOXO3A Monoclonal Antibody (CAB9270) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. RRID AB_2863703 Gene ID 2309 Swiss Prot Synonym FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
FOXO3A Monoclonal Antibody
SKU:
CAB9270
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC1505
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2
Positive Sample:
HeLa, PC-3, Mouse lung, Mouse brain
Cellular Localization:
Cytoplasm, Nucleus, Cytosol.
Calculated MW:
71kDa
Observed MW:
90kDa
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. RRID AB_2863703 Gene ID 2309 Swiss Prot Synonym FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Purification Method:
Affinity purification
Gene ID:
2309
RRID:
AB_2863703
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FOXO3A Rabbit mAb (CAB9270) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Western blot analysis of various lysates using FOXO3A Rabbit mAb (CAB9270) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
