The HIPK3 Polyclonal Antibody (PAC053922) is ideal for researchers studying the protein HIPK3, which plays a critical role in various cellular processes such as apoptosis, transcription regulation, and development. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to HIPK3, researchers can accurately detect and analyze the protein in different cell types, making it a valuable tool for studies in molecular biology and cancer research.
HIPK3, a member of the homeodomain-interacting protein kinase family, is involved in diverse physiological functions including cell proliferation, differentiation, and survival. Dysregulation of HIPK3 has been linked to various diseases, highlighting its potential as a therapeutic target for conditions like cancer, neurodegenerative disorders, and metabolic diseases. Investigating the role of HIPK3 in these pathological processes is essential for the development of targeted treatments that modulate its activity and improve patient outcomes.
Immunohistochemistry of paraffin-embedded human bladder cancer using PACO53922 at dilution of 1:100.
Immunofluorescent analysis of MCF-7 cells using PACO53922 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human colon cancer using PACO53922 at dilution of 1:100.
Background:
Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
Synonyms:
Homeodomain-interacting protein kinase 3 (EC 2.7.11.1) (Androgen receptor-interacting nuclear protein kinase) (ANPK) (Fas-interacting serine/threonine-protein kinase) (FIST) (Homolog of protein kinase YAK1), HIPK3, DYRK6 FIST3 PKY
UniProt Protein Function:
Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
