Histone H3K9me1 Antibody (PACO24574)
- SKU:
- PACO24574
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- IP
- ChIP
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | Histone H3K9me1 Antibody (PACO24574) |
| Antibody SKU: | PACO24574 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF, IP, ChIP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3 |
| Form: | Liquid |
| Storage Buffer: | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K9me1 antibody. |
 | Immunofluorescence analysis of 293T cell using H3K9me1 antibody. Blue: DAPI for nuclear staining. |
 | Dot-blot analysis of all sorts of methylation peptides using H3K9me1 antibody. |
| Background: | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). 1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79. 2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41. 3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5. 4. Cheung, P. et al. (2000) Cell 103, 263-71. 5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73. 6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9. 7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13. 8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60. 9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9. 10. Preuss, U. et al. (2003) Nucleic acid, Res 31, 878-85. 11. Dai, J. et al. (2005) Genes Dev 19, 472-88. |
| Synonyms: | HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3; |
| UniProt Protein Function: | HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1q42 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16695 |
| NCBI GenInfo Identifier: | 18202512 |
| NCBI Gene ID: | 8290 |
| NCBI Accession: | Q16695.3 |
| UniProt Secondary Accession: | Q16695,Q6FGU4, B2R5K3, |
| UniProt Related Accession: | Q16695 |
| Molecular Weight: | |
| NCBI Full Name: | Histone H3.1t |
| NCBI Synonym Full Names: | histone cluster 3, H3 |
| NCBI Official Symbol: | HIST3H3 |
| NCBI Official Synonym Symbols: | H3t; H3.4; H3/g; H3FT |
| NCBI Protein Information: | histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T |
| UniProt Protein Name: | Histone H3.1t |
| UniProt Synonym Protein Names: | H3/g |
| UniProt Gene Name: | HIST3H3 |
| UniProt Entry Name: | H31T_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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