The Hsp90alpha/beta Monoclonal Antibody (CAB5027) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] RRID AB_2863419 Gene ID 3320 3326 Swiss Prot Synonym EL52; HEL-S-65p; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp103; Hsp89; Hsp90; LAP-2; LAP2; Hsp90α/β
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Monkey samples.
Product Name:
Hsp90alpha/beta Monoclonal Antibody
SKU:
CAB5027
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat, Monkey
Clone Number:
ARC1169
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:5000 - 1:20000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa, K-562, A549, COS-7, NIH/3T3, C2C12, Mouse liver, Mouse brain, PC-12, C6, Rat liver, Rat brain
Cellular Localization:
Cell Membrane, Cytoplasm, Melanosome.
Calculated MW:
90kDa
Observed MW:
90kDa
The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] RRID AB_2863419 Gene ID 3320 3326 Swiss Prot Synonym EL52; HEL-S-65p; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp103; Hsp89; Hsp90; LAP-2; LAP2; Hsp90α/β
Purification Method:
Affinity purification
Gene ID:
3320 3326
RRID:
AB_2863419
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Hsp90α/β Rabbit mAb (CAB5027) at 1:11000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
