The Human AMY1 (Amylase Alpha 1) Salivary ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of AMY1 levels in human saliva samples. This kit provides reliable and reproducible results, making it suitable for various research applications.AMY1 is an important enzyme involved in the digestion of carbohydrates, breaking down starch into sugars. Variations in AMY1 levels have been linked to conditions such as obesity, diabetes, and gastrointestinal diseases, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
Overall, the Human AMY1 Salivary ELISA Kit is a valuable tool for researchers studying the role of AMY1 in health and disease, offering precise and reliable measurements to advance their research.
Product Name:
Human AMY1 (Amylase Alpha 1, Salivary) ELISA Kit
SKU:
HUES01543
Target:
Human AMY1 (Amylase Alpha 1, Salivary)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AMY1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AMY1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AMY1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AMY1. You can calculate the concentration of Human AMY1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
91-103
100-115
Average (%)
105
96
106
1:4
Range (%)
85-98
85-100
89-102
Average (%)
92
91
95
1:8
Range (%)
86-102
87-97
88-99
Average (%)
93
92
93
1:16
Range (%)
91-106
83-93
80-92
Average (%)
99
87
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.6
13.1
33.55
4.41
12.42
32.89
Standard deviation
0.24
0.76
1.48
0.28
0.7
1.07
C V (%)
5.22
5.8
4.41
6.35
5.64
3.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AMY1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AMY1 in samples. No significant cross-reactivity or interference between Human AMY1 and analogues was observed.
