The Human CTn-I (cTnI, TNNI3) Cardiac Troponin I ELISA Kit is specifically designed for the precise measurement of cardiac troponin I levels in human samples such as serum, plasma, and cell culture supernatants. With its outstanding sensitivity and specificity, this kit ensures accurate and consistent results, making it ideal for various research applications in the field of cardiology.Cardiac troponin I is a key biomarker for assessing cardiac health and diagnosing conditions such as myocardial infarction (heart attack) and other cardiovascular diseases. Monitoring CTn-I levels can help in early detection, risk stratification, and treatment evaluation in patients with heart-related issues. This ELISA kit provides researchers with a reliable tool for studying cardiovascular diseases and exploring potential treatment strategies.
Product Name:
Human cTn-I/TNNI3 (cardiac Troponin I) ELISA Kit
SKU:
HUES01810
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
