The Rat CTnI (cTnI3) Cardiac Troponin I ELISA Kit is a highly sensitive and reliable assay for the quantification of cardiac troponin I levels in rat serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it suitable for a wide range of research applications.Cardiac troponin I is a key biomarker for the diagnosis and monitoring of cardiac injury and myocardial infarction. Elevated levels of CTnI in the blood are indicative of heart muscle damage, making it an important marker for cardiovascular diseases. This ELISA kit allows for the precise measurement of CTnI levels, enabling researchers to study cardiac health and develop potential therapeutic interventions.
Product Name:
Rat cTn-I/TNNI3 (Cardiac Troponin I) ELISA Kit
SKU:
RTES00975
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
