Description
| Product Name: | Human DKK1 Hi-5 Recombinant Protein |
| Product Code: | RPPB3356 |
| Size: | 5µg |
| Species: | Human |
| Target: | DKK1 Hi-5 |
| Synonyms: | DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. |
| Source: | Hi-5 Baculovirus |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The DKK1 protein solution (0.25mg/ml) contains PBS (pH 7.4) and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPTLNSVLN SNAIKNLPPP LGGAAGHPGS AVSAAPGILY PGGNKYQTID NYQPYPCAED EECGTDEYCA SPTRGGDAGV QICLACRKRR KRCMRHAMCC PGNYCKNGIC VSSDQNHFRG EIEETITESF GNDHSTLDGY SRRTTLSSKM YHTKGQEGSV CLRSSDCASG LCCARHFWSK ICKPVLKEGQ VCTKHRRKGS HGLEIFQRCY CGEGLSCRIQ KDHHQASNSS RLHTCQRHSG RLVPRGSHHH HHH |
DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions and takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid and is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer.
DKK1 produced in Hi-5 is a single, glycosylated polypeptide chain containing 253 amino acids (32-266 a.a.) and having a molecular mass of 27.8 kDa (Molecular size on DSD-PAGE will appear at approximately 40kDa).DKK1 is expressed with a 15 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family. |
| UniProt Protein Details: | Protein type:Inhibitor; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 10q11.2 Cellular Component: early endosome membrane; extracellular region; extracellular space; plasma membrane Molecular Function:growth factor activity; low-density lipoprotein receptor binding; protein binding; receptor antagonist activity; signal transducer activity Biological Process: negative regulation of mesodermal cell fate specification; negative regulation of peptidyl-serine phosphorylation; negative regulation of protein binding; negative regulation of synaptogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of Wnt receptor signaling pathway; regulation of endodermal cell fate specification; regulation of receptor internalization |
| NCBI Summary: | This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O94907 |
| NCBI GenInfo Identifier: | 13124053 |
| NCBI Gene ID: | 22943 |
| NCBI Accession: | O94907.1 |
| UniProt Secondary Accession: | O94907,B2RC19, |
| UniProt Related Accession: | O94907 |
| Molecular Weight: | 28,672 Da |
| NCBI Full Name: | Dickkopf-related protein 1 |
| NCBI Synonym Full Names: | dickkopf WNT signaling pathway inhibitor 1 |
| NCBI Official Symbol: | DKK1�� |
| NCBI Official Synonym Symbols: | SK; DKK-1�� |
| NCBI Protein Information: | dickkopf-related protein 1 |
| UniProt Protein Name: | Dickkopf-related protein 1 |
| UniProt Synonym Protein Names: | SK |
| Protein Family: | Dickkopf-related protein |
| UniProt Gene Name: | DKK1�� |
| UniProt Entry Name: | DKK1_HUMAN |
