Description
| Product Name: | Human FNDC5 Recombinant Protein |
| Product Code: | RPPB3550 |
| Size: | 10µg |
| Species: | Human |
| Target: | FNDC5 |
| Synonyms: | Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2, Irisin, FRCP2, FNDC5. |
| Source: | Yeast |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The FNDC5 was lyophilized from 0.45 �m filtered solution in PBS. |
| Solubility: | It is recommended to reconstitute the lyophilized Fibronectin Type III Domain Containing 5 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Fibronectin Type III Domain Containing 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FNDC5 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | S_P__S__A__P__V__N__V__T__V__R__H__L__K__A__N__S__A__V__V__S_W__D__V__L__E__D__E__V__V__I__G__F__A__I__S__Q__Q__K__K__D _V__R__M__L__R__F__I__Q__E__V__N__T__T__T__R__S__C__A__L__W__ D__L__E__E__D__T__E__Y__I__V__H__V__Q__A__I__S__I__Q__G__Q_S__P__A__S__E__P__V__L__F__K__T__P__R__E__A__E__K__M__A__S_ K__N__K__D__E__V__T__M__K__E |
| Biological Activity: | The biological activity of recombinant human irisin was measured by the ability to induce UCP-1 expression of adipocytes. The specific activity is 10ng/ml. |
Fibronectin Type III Domain Containing 5 (Irisin) is a newly discovered hormone, secreted into circulation by muscle, which acts on white adipocytes and promotes WAT �browning�. r-Irisin can reduce body weight and cause induction of brown adipocyte in vivo, including a capacity for thermogenic energy expenditure mediated by uncoupling protein 1 (UCP-1). Irisin mature protein is comprised of 110 amino acid residues and two potential glycosylation sites. High levels of Irisin can be found in the heart whereas Very low expression is found in the colon, pancreas and spleen, if any.
Fibronectin Type III Domain Containing 5 Human Recombinant produced in yeast is a glycosylated, polypeptide chain containing 110 amino acids and having a molecular mass of 20-25 kDa.FNDC5 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 1p35.1 Cellular Component: endoplasmic reticulum; extracellular region; plasma membrane Biological Process: response to muscle activity |
| NCBI Summary: | This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
| UniProt Code: | Q8NAU1 |
| NCBI GenInfo Identifier: | 403314395 |
| NCBI Gene ID: | 252995 |
| NCBI Accession: | Q8NAU1.3 |
| UniProt Secondary Accession: | Q8NAU1,Q6P6D9, Q7Z676, A6NMC9, D3DPQ6, |
| UniProt Related Accession: | Q8NAU1 |
| Molecular Weight: | 23659 Da |
| NCBI Full Name: | Fibronectin type III domain-containing protein 5 |
| NCBI Synonym Full Names: | fibronectin type III domain containing 5 |
| NCBI Official Symbol: | FNDC5�� |
| NCBI Official Synonym Symbols: | FRCP2; irisin�� |
| NCBI Protein Information: | fibronectin type III domain-containing protein 5 |
| UniProt Protein Name: | Fibronectin type III domain-containing protein 5 |
| UniProt Synonym Protein Names: | Fibronectin type III repeat-containing protein 2 |
| Protein Family: | Fibronectin type III domain-containing protein |
| UniProt Gene Name: | FNDC5�� |
| UniProt Entry Name: | FNDC5_HUMAN |
