Human GABARAPL1 Recombinant Protein (RPPB3568)
- SKU:
- RPPB3568
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9H0R8
Description
| Product Name: | Human GABARAPL1 Recombinant Protein |
| Product Code: | RPPB3568 |
| Size: | 20µg |
| Species: | Human |
| Target: | GABARAPL1 |
| Synonyms: | Gamma-aminobutyric acid receptor-associated protein-like 1, Early estrogen-regulated protein, GABA(A) receptor-associated protein-like 1, Glandular epithelial cell protein 1, GEC-1, GABARAPL1, GEC1, ATG8, APG8L, ATG8B, ATG8L, APG8-LIKE. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GABARAPL1 solution (1 mg/ml) contains 20mM Tris buffer (pH 8.0), 10% glycerol, 1mM DTT and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MKFQYKEDHP FEYRKKEGEK IRKKYPDRVP VIVEKAPKAR VPDLDKRKYL VPSDLTVGQF YFLIRKRIHL RPEDALFFFV NNTIPPTSAT MGQLYEDNHE EDYFLYVAYS DESVYGK |
GABARAPL1 [Gamma-aminobutyric acid (GABA) receptor-associated protein-like 1] is a member of the MAP1 LC3 family. GABARAPL1 increases cell surface expression of kappa type opioid receptor through facilitating anterograde intracellular trafficking of the receptor.
GABARAPL1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 137 amino acids (1-117 a.a.) and having a molecular mass of 16.2kDa (Molecular size on SDS-PAGE will appear higher). The GABARAPL1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GABARAPL1: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Belongs to the MAP1 LC3 family. Interacts with GABRG2 and beta-tubulin. Interacts with KOR-1. |
| UniProt Protein Details: | Protein type:Vesicle; Adaptor/scaffold; Microtubule-binding; Autophagy; Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 12p13.2 Cellular Component: autophagic vacuole; cytoplasmic vesicle membrane; cytosol; dendrite cytoplasm; endoplasmic reticulum; Golgi apparatus; intracellular; microtubule; mitochondrion Molecular Function:beta-tubulin binding; GABA receptor binding; protein binding; Tat protein binding; ubiquitin protein ligase binding Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; macroautophagy; mitochondrion degradation |
| UniProt Code: | Q9H0R8 |
| NCBI GenInfo Identifier: | 13899219 |
| NCBI Gene ID: | 23710 |
| NCBI Accession: | NP_113600.1 |
| UniProt Secondary Accession: | Q9H0R8,Q6FIE6, B4E0Y7, |
| UniProt Related Accession: | Q9H0R8 |
| Molecular Weight: | 17,008 Da |
| NCBI Full Name: | gamma-aminobutyric acid receptor-associated protein-like 1 |
| NCBI Synonym Full Names: | GABA type A receptor associated protein like 1 |
| NCBI Official Symbol: | GABARAPL1�� |
| NCBI Official Synonym Symbols: | ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE�� |
| NCBI Protein Information: | gamma-aminobutyric acid receptor-associated protein-like 1 |
| UniProt Protein Name: | Gamma-aminobutyric acid receptor-associated protein-like 1 |
| UniProt Synonym Protein Names: | Early estrogen-regulated protein; GABA(A) receptor-associated protein-like 1; Glandular epithelial cell protein 1; GEC-1 |
| Protein Family: | Gamma-aminobutyric acid receptor-associated protein |
| UniProt Gene Name: | GABARAPL1�� |
| UniProt Entry Name: | GBRL1_HUMAN |
