Human GADD45A Recombinant Protein (RPPB3570)
- SKU:
- RPPB3570
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P24522
Description
| Product Name: | Human GADD45A Recombinant Protein |
| Product Code: | RPPB3570 |
| Size: | 25µg |
| Species: | Human |
| Target: | GADD45A |
| Synonyms: | Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein, DDIT-1, GADD45A, DDIT1, GADD45. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The GADD45A protein solution contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA ADEDDDRDVA LQIHFTLIQA FCCENDINILRVSNPGRLAE LLLLETDAGP AASEGAEQPP DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPERLEHHH HHH |
Growth arrest and DNA Damage-Inducible Protein (GADD45A) binds both Cdks and PCNA. GADD45A is involved in DNA replication and repair. GADD45A stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A may serve as a link between p53-dependent cell cycle checkpoint and DNA repair. The GADD45A gene belongs to a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. GADD45A binds to proliferating cell nuclear antigen.
GADD45A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids (1-165 a.a.) and having a molecular mass of 19.4kDa.GADD45A is fused to 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; DNA repair, damage; Apoptosis; Cell cycle regulation Chromosomal Location of Human Ortholog: 1p31.2 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: activation of MAPKKK activity; apoptosis; cell cycle arrest; DNA damage response, signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; positive regulation of apoptosis; positive regulation of JNK cascade; regulation of cyclin-dependent protein kinase activity |
| NCBI Summary: | This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010] |
| UniProt Code: | P24522 |
| NCBI GenInfo Identifier: | 120751 |
| NCBI Gene ID: | 1647 |
| NCBI Accession: | P24522.1 |
| UniProt Secondary Accession: | P24522,Q5TCA7, Q5TCA8, |
| UniProt Related Accession: | P24522 |
| Molecular Weight: | 14,622 Da |
| NCBI Full Name: | Growth arrest and DNA damage-inducible protein GADD45 alpha |
| NCBI Synonym Full Names: | growth arrest and DNA damage inducible alpha |
| NCBI Official Symbol: | GADD45A�� |
| NCBI Official Synonym Symbols: | DDIT1; GADD45�� |
| NCBI Protein Information: | growth arrest and DNA damage-inducible protein GADD45 alpha |
| UniProt Protein Name: | Growth arrest and DNA damage-inducible protein GADD45 alpha |
| UniProt Synonym Protein Names: | DNA damage-inducible transcript 1 protein; DDIT-1 |
| Protein Family: | Growth arrest and DNA damage-inducible protein |
| UniProt Gene Name: | GADD45A�� |
| UniProt Entry Name: | GA45A_HUMAN |
