ELISA Kits
Standard curve of Human OCLN ELISA Kit
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ELISA by Technology
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ELISA by Species
At Assay Genie we support a wide range of researchers focussing on key areas of veterinary, immunology, vaccine and neuroscience research through our range of pre-coated ELISA kits for over 20 species and key DIY ELISA kits.
Preclinical Animal
Large Animal
ELISA Features & Benefits
| Validated | Extensive validation data including Spike-recovery, Linearity, Range, CV%, Standard Curves | |
| Sensitive | Pre-coated 96-well ELISA kits with picogram sensitivity for many analytes | |
| Versatile | Compatible with plasma, serum, cell culture media, lysates & clarified body fluids | |
| Specific | Tested for cross-reactivity |
ELISA Kit Promotion
ELISA Kit Example Data
Spike & Recovery
In order to asses the accuracy of an ELISA kit, spike and recovery and linear of dilution experiments provide important information in the validation and assessment of the accuracy of the ELISA kit assay.
The use of spike and recovery allows researchers to determine whether the detection of their analyte of interest is affected by the standard diluent and the biological sample matrix. In a spike and recovery experiment to assess and validate and ELISA kit a known amount of analyte (recombinant protein or hormone) is added into a test sample matrix and the response is compared to the identical spike into the standard diluent.
The example below of matrices listed below were spiked with certain level of IL8 and the recovery rates were calculated by comparing the measured value to the expected amount of IL8 in samples.
| Matrix | Recovery range(%) | Average(%) |
|
Serum(n=5) |
88-101 |
95 |
|
EDTA plasma(n=5) |
90-105 |
99 |
|
UFH plasma(n=5) |
88-103 |
96 |
Linearity
In order to assess the precision for samples tested at different dilutions a linearity of dilution experiment is carried out. If the linearity of the assay is good over a range of dilutions (i.e. 1:2 to 1:16 for example), then the assay provides the ability to assay a sample with different levels of analyte. If a sample contains a high level of analyte, a sample may be diluted with standard dilution buffer and allow for comparison with an undiluted sample that contains low levels of the analyte of interest.
In the example below the linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
|
Serum(n=5) |
91-102% |
87-101% |
89-98% |
92-104% |
|
EDTA plasma(n=5) |
89-100% |
88-98% |
83-92% |
84-96% |
|
UFH plasma(n=5) |
83-96% |
80-96% |
80-100% |
81-100% |
Coefficient of Variability (% CV)
ELISA kits are great tools to allow researchers to quantifiably measure the amount of analyte in a sample. However, in order to assess and show how well and ELISA assay kit is ran the results of the assay must a low coefficient of variability (%CV or CV). Whereby the %CV statistically shows the variance between replicate samples (Intra-assay) in an assay or between separately run assays (Inter-assay).
| Analysis | % CV |
|
Intra-assay |
<8% |
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Inter-assay |
<10% |
PharmaGenie ELISA Kits
PharmaGenie ELISA Kits from Assay Genie are high quality ELISA Kits designed to meet the needs of pharma and biotech research. Focussing on high quality monoclonal antibody pairs & reagents that have been been validated according ISO 9001:2000 quality systems, PharmaGenie ELISA Kits are excellent assays to help discover our future.
High Quality Monoclonal Pairs
Control of our own mAb development coupled with robust manufacturing procedures ensures excellent performance and batch to batch consistency.
High Sensitivity Assays
High Sensitivity (HS) kits for the detection of low expressing proteins with sensitivities below 1pg/ml.
PharmaGenie ELISA Features & Benefits
| Highly sensitve assay | |
| Fast procedure | |
| Accuracy and reliability are guaranteed as all our reagents have been validated according to ISO 9001:2000 quality systems | |
| Recognises both Natural and Recombinant antigen Specificity | |
| No cross reactivity with other human cytokines tested | |
| Standard Calibration to NIBSC |
Cytokine ELISA kits
Human Cytokine ELISA Kits
At Assay Genie we provide a huge range of cytokine ELISA kits for human, mouse, rat and many more species. Our range of cytokine ELISA kits cover key Interleukins, Interferons, TNF family members and more. Further Assay Genie provide a small subset range of high sensitivity cytokine ELISA kits.
High Sensitivity Cytokine ELISA kits
Our PharmaGenie high sensitivity cytokine ELISA kits use high quality monoclonal antibodies to detect Interleukin 2 to 12 and IFN-gamma at less than <1pg/mL. The high sensitivity ELISA kits are a fast protocol that allows researchers to get the results they need with a protocol of 3 hours.
Cytokine ELISA Features & Benefits
| Less than 1pg/mL sensitivity | |
| Key Interleukins | |
| Fast protocol of ~3 hours | |
| Ship at room temperature and eco-friendly |
DIY Development ELISA Kits (antibody pairs)
SuperSet Development ELISA kits allow researchers to prepare and plate their own ELISA plates using antibody pairs. SuperSet Development ELISA kits from Assay Genie are high quality ELISA kits with sensitivity of <5pg/ml. These kits are made with high quality monoclonal antibodies to allow for increased sensitivity and specificity.
Features & Benefits
| Highly optimised monoclonal antibody pair | |
| High quality purified recombinant protein to generate consistent standard curves | |
| Reagents have been validated according ISO 9001:2000 quality systems | |
| Natural and Recombinant antigen Specificity | |
| No cross reactivity with other cytokines tested | |
| Standard Calibration to NIBSC | |
| ELISA Kits developed with pharmaceutical and biotech research sectors in mind |
DIY Development ELISA Kit Protocol Schematic
Figure: SuperSet developemt ELISA kits protocol. Coats wells with antibody & incubate overnight at 4'C. Add blocking buffer. Incubate for 120 mins at room temperature. Wash wells. Add standard and samples, add Biotin detection working solution. Incubate for 60 mins at RT. Wash wells. Add Step.-HRP. Wash wells. Add TMB substrate & incubate for 5-15 mins. Add stop solution and read at 450nm.
Therapeutic Drug Monitoring ELISA Kits
The Assay Genie Therapeutic Antibody, Biosimilar & Anti-Drug Antibody (ADA) ELISA kits are a premium range of kits designed to detect free therapeutic antibodies, biosimilars and Anti-Drug Antibodies in serum & plasma samples.
These kits use the highest quality components and are manufactured under ISO 13485 quality systems according to CE IVD standards.
Premium Quality Validated Assays
- Manufactured in Europe under ISO 13485 quality systems & according to CE IVD standards.
- Kits for Research-Use-Only.
- Inter and intra assay CV meet requirements for FDA & EMEA
Validation, verification and lot release performed according to best-in-class scientific guidelines and literature
Quantitative & Qualitative Screening Kits
- Quantitative ELISA kits for the measurement of the World’s leading
therapeutic antibodies & biosimilars - Qualitative kits enabling the screening of deleterious ADAs
- Dual kits allowing for the screening of ADAs and free therapeutic antibodies
Optimized Format & Protocol
- Use as little as 10ul sample per well – excellent for clinical, mouse, rat & primate samples
- Get your results in as little as 55 minutes for some kits
- All kits shipped at room temperature