Description
| Product Name: | Human MEMO1 Recombinant Protein |
| Product Code: | RPPB3956 |
| Size: | 20µg |
| Species: | Human |
| Target: | MEMO1 |
| Synonyms: | Protein MEMO1, C21orf19-like protein, Hepatitis C virus NS5A-transactivated protein 7, HCV NS5A-transactivated protein 7, Mediator of ErbB2-driven cell motility 1, Mediator of cell motility 1, Memo-1, MEMO1, C2orf4, MEMO, NS5ATP7, CGI-27. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | MEMO1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer, pH8.0, 50% glycerol, 5mM DTT, 300mM NaCl and 2mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMMSNRV VCREASHAGS WYTASGPQLN AQLEGWLSQV QSTKRPARAI IAPHAGYTYC GSCAAHAYKQ VDPSITRRIF ILGPSHHVPL SRCALSSVDI YRTPLYDLRI DQKIYGELWK TGMFERMSLQ TDEDEHSIEM HLPYTAKAME SHKDEFTIIP VLVGALSESK EQEFGKLFSK YLADPSNLFV VSSDFCHWGQ RFRYSYYDES QGEIYRSIEH LDKMGMSIIE QLDPVSFSNY LKKYHNTICG RHPIGVLLNA ITELQKNGMN MSFSFLNYAQ SSQCRNWQDS SVSYAAGALT VH |
Mediator of ErbB2-driven cell motility 1 (MEMO1) is a member of the UPF0103 family. MEMO1 regulates cell migration by transmitting extracellular chemotactic signals to the microtubule cytoskeleton. Furthermore, MEMO1 controls the localization of APC and CLASP2 to the cell membrane, using the regulation of GSK3B activity. MEMO1 is essential for breast carcinoma cell migration, suggesting a key role in tumorigenesis. MEMO1 is also a mediator of ERBB2 signaling.
MEMO1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 322 amino acids (1-297 a.a) and having a molecular mass of 36.4kDa.MEMO1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MEMO1: a highly conserved protein that controls cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Regulates directionality during HER2-dependent cell migration. Controls microtubules, the actin network, and adhesion site formation in migrating cells via the MEMO1-RHOA-DIAPH1 signaling pathway. Homologous to class III nonheme iron-dependent dioxygenases. No metal-binding or enzymatic activity has been detected. Binds HER2 phosphorylated at Y1222 via its vestigial enzymatic active site. Binds to and influences insulin-like growth factor-I (IRS1) receptor-dependent signaling events in breast carcinogenesis. Influences the epithelial-mesenchymal transition (EMT) via the IRS1/PI3K/Akt/Snail1 pathway. Interacts with cofilin, and influences PLCg1 and cofilin activities. Increased expression is associated with cancer aggressiveness. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Two isoform of the human protein are produced by alternatuve splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2p22-p21 Cellular Component: cytosol; nucleus Molecular Function:protein binding Biological Process: regulation of microtubule-based process |
| UniProt Code: | Q9Y316 |
| NCBI GenInfo Identifier: | 17511761 |
| NCBI Gene ID: | 51072 |
| NCBI Accession: | BC018733 |
| UniProt Secondary Accession: | Q9Y316,Q5R2V8, Q5R2V9, Q6NSL5, B4DLS0, D6W575, |
| UniProt Related Accession: | Q9Y316 |
| Molecular Weight: | 34,294 Da |
| NCBI Full Name: | Homo sapiens mediator of cell motility 1, mRNA |
| NCBI Synonym Full Names: | mediator of cell motility 1 |
| NCBI Official Symbol: | MEMO1�� |
| NCBI Official Synonym Symbols: | MEMO; C2orf4; CGI-27; NS5ATP7�� |
| NCBI Protein Information: | protein MEMO1 |
| UniProt Protein Name: | Protein MEMO1 |
| UniProt Synonym Protein Names: | C21orf19-like protein; Hepatitis C virus NS5A-transactivated protein 7; HCV NS5A-transactivated protein 7; Mediator of ErbB2-driven cell motility 1; Mediator of cell motility 1; Memo-1 |
| Protein Family: | MEMO1 family protein |
| UniProt Gene Name: | MEMO1�� |
| UniProt Entry Name: | MEMO1_HUMAN |
