Human NQO2 Recombinant Protein (RPPB2036)
- SKU:
- RPPB2036
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P16083
- Research Area:
- Enzymes
Description
Product Name: | Human NQO2 Recombinant Protein |
Product Code: | RPPB2036 |
Size: | 25µg |
Species: | Human |
Target: | NQO2 |
Synonyms: | DHQV, DIA6, QR2, EC 1.10.99.2, NMOR2, NQO2, NRH:quinone oxidoreductase 2, NRH dehydrogenase [quinone] 2, Ribosyldihydronicotinamide dehydrogenase [quinone]. |
Source: | Escherichia Coli |
Physical Appearance: | Sterile filtered colorless solution. |
Formulation: | NQO2 Human solution (1mg/ml) containing 20mM Tris-HCl pH-8, 1mM DTT & 10% glycerol. |
Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
Purity: | Greater than 95% as determined by SDS-PAGE. |
Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAGKKVLIVY AHQEPKSFNG SLKNVAVDEL SRQGCTVTVS DLYAMNFEPR ATDKDITGTL SNPEVFNYGV ETHEAYKQRS LASDITDEQK KVREADLVIF QFPLYWFSVP AILKGWMDRV LCQGFAFDIP GFYDSGLLQG KLALLSVTTG GTAEMYTKTG VNGDSRYFLW PLQHGTLHFC GFKVLAPQIS FAPEIASEEE RKGMVAAWSQ RLQTIWKEEP IPCTAHWHFG Q |
NQO2 is a flavoprotein that catalyzes the 2-electron reduction of diverse quinones, redox dyes, and the vitamin K menadione. NQO2 mainly uses dihydronicotinamide riboside (NRH) as the electron donor. NQO2 catalyzes the metabolic detoxification of quinones and their derivatives to hydroquinones. This detoxification process protects cells against quinone-induced oxidative stress, cytotoxicity and mutagenicity.
NQO2 Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 251amino acids (1-231 a.a.) and having a molecular mass of 28.1 kDa. NQO2 protein is fused to a 20 amino acid His-Tag at N-terminus and purified by standard chromatography.
UniProt Protein Function: | NQO2: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Belongs to the NAD(P)H dehydrogenase (quinone) family. |
UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.10.99.2 Chromosomal Location of Human Ortholog: 6p25.2 Cellular Component: nucleoplasm; cytoplasm Molecular Function:electron carrier activity; dihydronicotinamide riboside quinone reductase activity; NADPH dehydrogenase (quinone) activity; metal ion binding Biological Process: memory Disease: Breast Cancer |
NCBI Summary: | This gene encodes a member of the thioredoxin family of enzymes. It is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone substrates and uses dihydronicotinamide riboside as a reducing coenzyme. Mutations in this gene have been associated with neurodegenerative diseases and several cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
UniProt Code: | P16083 |
NCBI GenInfo Identifier: | 317373581 |
NCBI Gene ID: | 4835 |
NCBI Accession: | P16083.5 |
UniProt Secondary Accession: | P16083,Q9JI75, |
UniProt Related Accession: | P16083 |
Molecular Weight: | 231 |
NCBI Full Name: | Ribosyldihydronicotinamide dehydrogenase |
NCBI Synonym Full Names: | NAD(P)H dehydrogenase, quinone 2 |
NCBI Official Symbol: | NQO2�� |
NCBI Official Synonym Symbols: | QR2; DHQV; DIA6; NMOR2�� |
NCBI Protein Information: | ribosyldihydronicotinamide dehydrogenase [quinone]; ribosyldihydronicotinamide dehydrogenase [quinone]; quinone reductase 2; NRH:quinone oxidoreductase 2; NAD(P)H menadione oxidoreductase-1, dioxin-inducible-2 |
UniProt Protein Name: | Ribosyldihydronicotinamide dehydrogenase [quinone] |
UniProt Synonym Protein Names: | NRH dehydrogenase [quinone] 2; NRH:quinone oxidoreductase 2; Quinone reductase 2; QR2 |
Protein Family: | NADH-quinone oxidoreductase |
UniProt Gene Name: | NQO2�� |
UniProt Entry Name: | NQO2_HUMAN |