Human Protein Kinase A regulatory subunit-1 alpha Recombinant Protein (CPRT2616)

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Data SheetMSDS

PKAR-I alpha Human Recombinant Protein

Name:PKAR-I alpha Human
Synonyms:cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1, TSE1, PRKAR1A PKR1, PRKAR1, CAR, CNC, CNC1, PKR1, ADOHR, PPNAD1, ACRDYS1.
Introduction:The Regulatory (R) subunit of Protein Kinase A (PKA) inhibits its kinase activity by shieldingthe Catalytic (C) subunit from physiological substrates. This inhibition is reversed in response to extra–cellular signals that increase cAMP levels in the cytoplasm. Upon cAMP bindingto R, C is allosterically released from R, activatinga spectrum of downstream signalingcascades. Crystallographic data indicated that a series of distinct conformational changes within CBD–A must occur to relay the cAMP signal from the cAMP bindingsite to the R:C interaction interface. One critical cAMP relay site within the CBD–A of R has been identified as Asp170 because the D170A mutation selectively reduces the negative cooperativity between the cAMP– and C–recognition sites (i.e. the KD for the R:C complex in the presence of cAMP is reduced by more than 12–fold), without significantly compromisingthe high affinity of R for both bindingpartners.
Description:PKA regulatory subunit I a Human Recombinant is a dimeric 86kDa protein (the monomer is 381 aa 43kDa). PKAR–I alpha is purified by proprietary chromatographic techniques.
Source:Escherichia Coli.
Pack size:25µg
Physical Appearance:Sterile Filtered clear solution.
Formulation:PKA regulatory subunit–I alpha is supplied at a concentration of 0.8 mg/ml in 20mM MOPS (pH 7.0), 150mM NaCl, 1mM 2–mercaptoethanol and 50% glycerol.
Stability:PKAR–Ia should be stored at 4°C if entire vial will be used within 2–4 weeks. For longterm storage it is recommended to store at –20°C. Avoid multiple freeze–thaw cycles.
Purity:Greater than 95% as determined by SDS–PAGE.
Amino Acid Sequence:N/A
Biological Activity:N/A
Usage:Assay Genie’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Additional Information

Product type:
Recombinant Protein
Host Species:
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