Human RHEB Recombinant Protein (RPPB4457)
- SKU:
- RPPB4457
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q15382
Description
| Product Name: | Human RHEB Recombinant Protein |
| Product Code: | RPPB4457 |
| Size: | 50µg |
| Species: | Human |
| Target: | RHEB |
| Synonyms: | Ras homolog enriched in brain, RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, RheB, Ras homolog enriched in brain GTP binding protein Rheb, Ras homolog enriched in brain 2, RHEB 2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The RHEB protein solution (1mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MASMTGGQQM GRGSASMPQS KSRKIAILGY RSVGKSSLTI QFVEGQFVDS YDPTIENTFT KLITVNGQEY HLQLVDTAGQ DEYSIFPQTY SIDINGYILV YSVTSIKSFE VIKVIHGKLL DMVGKVQIPI MLVGNKKDLH MERVISYEEG KALAESWNAA FLESSAKENQ TAVDVFRRII LEAEKMDGAA SQGKSSC |
RHEB is part of the Ras & GTPase superfamily that was originally identified as an immediate-early gene in brain but is also widely expressed in other tissues. RHEB encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RHEB is necessary in regulation of growth and cell cycle progression due to its role in the TOR/S6K signaling pathway. RHEB has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. RHEB induces oncogenic transformation. RHEB overexpression accelerates lymphomagenesis and is associated with prostate cancer. RHEB can cytopathologically distinguish between fibroadenoma from malignant breast carcinomas which is considered as a secondary diagnostic tool. RHEB has a central role in the regulation of the Ras/B-Raf/C-Raf/MEK signaling network.
RHEB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 197 amino acids (1-181 amino acids) and having a molecular mass of 21.7 kDa.The RHEB is fused to T7-tag at N-terminus (16 a.a.) and is purified by standard chromatography techniques.
| UniProt Protein Function: | RHEB: Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2. Ubiquitous. Highest levels observed in skeletal and cardiac muscle. Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2. Belongs to the small GTPase superfamily. Rheb family. |
| UniProt Protein Details: | Protein type:G protein; G protein, monomeric; G protein, monomeric, Rheb Chromosomal Location of Human Ortholog: 7q36 Cellular Component: Golgi membrane; spliceosome; endoplasmic reticulum membrane; membrane; cell soma; dendrite; plasma membrane; cytosol Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; metal ion binding; protein kinase binding Biological Process: metabolic process; small GTPase mediated signal transduction; insulin receptor signaling pathway; signal transduction; cell cycle arrest; positive regulation of TOR signaling pathway |
| NCBI Summary: | This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15382 |
| NCBI GenInfo Identifier: | 6919957 |
| NCBI Gene ID: | 6009 |
| NCBI Accession: | Q15382.1 |
| UniProt Secondary Accession: | Q15382,Q53Y56, Q99444, B3KWN6, D3DX13, |
| UniProt Related Accession: | Q15382 |
| Molecular Weight: | 184 |
| NCBI Full Name: | GTP-binding protein Rheb |
| NCBI Synonym Full Names: | Ras homolog enriched in brain |
| NCBI Official Symbol: | RHEB�� |
| NCBI Official Synonym Symbols: | RHEB2�� |
| NCBI Protein Information: | GTP-binding protein Rheb; Ras homolog enriched in brain 2 |
| UniProt Protein Name: | GTP-binding protein Rheb |
| UniProt Synonym Protein Names: | Ras homolog enriched in brain |
| Protein Family: | Rheb-like protein |
| UniProt Gene Name: | RHEB�� |
| UniProt Entry Name: | RHEB_HUMAN |
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