Human UBA5 Recombinant Protein (RPPB2364)
- SKU:
- RPPB2364
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9GZZ9
- Research Area:
- Enzymes
Description
| Product Name: | Human UBA5 Recombinant Protein |
| Product Code: | RPPB2364 |
| Size: | 10µg |
| Species: | Human |
| Target: | UBA5 |
| Synonyms: | Ubiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme 5, ThiFP1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1, UBA5, UBE1DC1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The UBA5 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 50mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMAESVE RLQQRVQELE RELAQERSLQ VPRSGDGGGG RVRIEKMSSE VVDSNPYSRL MALKRMGIVS DYEKIRTFAV AIVGVGGVGS VTAEMLTRCG IGKLLLFDYD KVELANMNRL FFQPHQAGLS KVQAAEHTLR NINPDVLFEV HNYNITTVENFQHFMDRISN GGLEEGKPVD LVLSCVDNFE ARMTINTACN ELGQTWMESG VSENAVSGHI QLIIPGESAC FACAPPLVVA ANIDEKTLKR EGVCAASLPT TMGVVAGILV QNVLKFLLNF GTVSFYLGYN AMQDFFPTMS MKPNPQCDDR NCRKQQEEYK KKVAALPKQE VIQEEEEIIH EDNEWGIELV SEVSEEELKN FSGPVPDLPE GITVAYTIPK KQEDSVTELT VEDSGESLED LMAKMKNM |
Ubiquitin-like modifier activating enzyme 5 (UBA5) is a member of the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are recognized as covalently conjugated to various cellular substrates by a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) has a vital role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 activates an ubiquitin-like protein, ubiquitin-fold modifier 1 (Ufm1), by forming a high-energy thioester bond. UBA5 is located primarily in cytoplasm, while it generally localizes to the nucleus in presence of SUMO2.
UBA5 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 428 amino acids (1-404) and having a molecular mass of 47.4kDa.UBA5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | E1-like enzyme which activates UFM1 and SUMO2. |
| NCBI Summary: | This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1. [provided by RefSeq, Feb 2016] |
| UniProt Code: | Q9GZZ9 |
| NCBI GenInfo Identifier: | 13376212 |
| NCBI Gene ID: | 79876 |
| NCBI Accession: | NP_079094 |
| UniProt Secondary Accession: | Q9GZZ9,Q96ST1, A6NJL3, D3DNC8, |
| UniProt Related Accession: | Q9GZZ9 |
| Molecular Weight: | 47.4kDa (428aa) confirmed by MALDI-TOF |
| NCBI Full Name: | ubiquitin-like modifier-activating enzyme 5 isoform 1 |
| NCBI Synonym Full Names: | ubiquitin like modifier activating enzyme 5 |
| NCBI Official Symbol: | UBA5�� |
| NCBI Official Synonym Symbols: | EIEE44; SCAR24; THIFP1; UBE1DC1�� |
| NCBI Protein Information: | ubiquitin-like modifier-activating enzyme 5 |
| UniProt Protein Name: | Ubiquitin-like modifier-activating enzyme 5 |
| UniProt Synonym Protein Names: | ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1 |
| UniProt Gene Name: | UBA5�� |
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