Description
| Product Name: | Human UBE2T Recombinant Protein |
| Product Code: | RPPB2391 |
| Size: | 25µg |
| Species: | Human |
| Target: | UBE2T |
| Synonyms: | EC 6.3.2.19, HSPC150, PIG50, Ubiquitin-conjugating enzyme E2 T, Ubiquitin-protein ligase T, Ubiquitin carrier protein T, Cell proliferation-inducing gene 50 protein, UBE2T. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear colorless solution. |
| Formulation: | The UBE2T solution contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDVLEH HHHHH |
UBE2T is part of the E2 ubiquitin-conjugating enzyme family that participates in the protein degradation pathway. UBE2T catalyzes the ATP-dependent attachment of ubiquitin to target proteins, thus tagging them for subsequent destruction by the proteasome. UBE2T is an important factor of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway.
UBE2T Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 205 amino acids (1-197 a.a.) and having a molecular mass of 23.6 kDa. The UBE2T is fused to an 8 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UBE2T: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; EC 6.3.2.19; Ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; chromatin binding; ATP binding; ligase activity Biological Process: protein monoubiquitination; protein autoubiquitination; DNA repair; response to DNA damage stimulus Disease: Fanconi Anemia, Complementation Group T |
| NCBI Summary: | The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9NPD8 |
| NCBI GenInfo Identifier: | 73622065 |
| NCBI Gene ID: | 29089 |
| NCBI Accession: | Q9NPD8.1 |
| UniProt Secondary Accession: | Q9NPD8,Q2TU36, |
| UniProt Related Accession: | Q9NPD8 |
| Molecular Weight: | 197 |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 T |
| NCBI Synonym Full Names: | ubiquitin-conjugating enzyme E2T |
| NCBI Official Symbol: | UBE2T�� |
| NCBI Official Synonym Symbols: | PIG50; HSPC150�� |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 T; ubiquitin-protein ligase T; ubiquitin carrier protein T; ubiquitin conjugating enzyme; cell proliferation-inducing gene 50 protein; ubiquitin-conjugating enzyme E2T (putative); HSPC150 protein similar to ubiquitin-conjugating enzyme |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 T |
| UniProt Synonym Protein Names: | Cell proliferation-inducing gene 50 protein; Ubiquitin carrier protein T; Ubiquitin-protein ligase T |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2T�� |
| UniProt Entry Name: | UBE2T_HUMAN |
