Anti-JAK3 Antibody (RACO0373)
- SKU:
- RACO0373
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
| Product Name: | JAK3 Antibody |
| Product SKU: | RACO0373 |
| Size: | 50ul |
| Host Species: | Homo sapiens (Human) |
| Tested Applications: | ELISA, IHC |
| Recommended Dilutions: | IHC:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | A synthesized peptide derived from human JAK3 |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Affinity-chromatography |
| Clonality: | Monoclonal |
| Isotype: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| Background: | Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion. |
| Synonyms: | Tyrosine-protein kinase JAK3 (EC 2.7.10.2) (Janus kinase 3) (JAK-3) (Leukocyte janus kinase) (L-JAK), JAK3 |
 | IHC image of RACO0373 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| UniProt Protein Function: | JAK3: a non-receptor tyrosine-kinase of the Jak family expressed predominantly in immune cells. Mediates signal transduction via the common gamma-chain of cytokine receptors, including IL-2, -4, -7, -9, and -15. Interacts with members of the STAT (signal transduction and activators of transcription) family. Interacts with STAM2 and SHB. Contains two protein kinase domains. Mutations that abrogate JAK3 function cause an autosomal SCID (severe combined immunodeficiency disease). Inhibitor: R017s for organ transplants. Three differentially spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; JakA family Chromosomal Location of Human Ortholog: 19p13.1 Cellular Component: cytoskeleton; cytosol; endomembrane system; extrinsic to internal side of plasma membrane Molecular Function:ATP binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein phosphatase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding Biological Process: adaptive immune response; B cell differentiation; cell migration; elevation of cytosolic calcium ion concentration; enzyme linked receptor protein signaling pathway; innate immune response; MAPKKK cascade; negative regulation of dendritic cell cytokine production; negative regulation of FasL biosynthetic process; negative regulation of interleukin-10 production; negative regulation of interleukin-12 production; negative regulation of T cell activation; negative regulation of T-helper 1 cell differentiation; peptidyl-tyrosine phosphorylation; positive regulation of activated T cell proliferation; positive regulation of calcium ion transport; positive regulation of GTPase activity; positive regulation of immune response; positive regulation of T cell proliferation; protein amino acid phosphorylation; STAT protein nuclear translocation; T cell homeostasis; tyrosine phosphorylation of STAT protein; tyrosine phosphorylation of Stat5 protein Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-negative |
| NCBI Summary: | The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008] |
| UniProt Code: | P52333 |
| NCBI GenInfo Identifier: | 50403745 |
| NCBI Gene ID: | 3718 |
| NCBI Accession: | P52333.2 |
| UniProt Secondary Accession: | P52333,Q13259, Q13260, Q13611, Q8N1E8, Q99699, Q9Y6S2 |
| UniProt Related Accession: | P52333 |
| Molecular Weight: | |
| NCBI Full Name: | Tyrosine-protein kinase JAK3 |
| NCBI Synonym Full Names: | Janus kinase 3 |
| NCBI Official Symbol: | JAK3 |
| NCBI Official Synonym Symbols: | JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN |
| NCBI Protein Information: | tyrosine-protein kinase JAK3 |
| UniProt Protein Name: | Tyrosine-protein kinase JAK3 |
| UniProt Synonym Protein Names: | Janus kinase 3; JAK-3; Leukocyte janus kinase; L-JAK |
| Protein Family: | Tyrosine-protein kinase |
| UniProt Gene Name: | JAK3 |
| UniProt Entry Name: | JAK3_HUMAN |
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