The KLF1 Antibody (CAB10581) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. RRID AB_2758122 Gene ID 10661 Swiss Prot Synonym EKLF; EKLF/KLF1; KLF1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
KLF1 Antibody
SKU:
CAB10581
Size:
100μL, 20μL
Reactivity:
Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EKLF, EKLF/KLF1, KLF1
Positive Sample:
Rat plasma
Cellular Localization:
Nucleus.
Calculated MW:
38kDa
Observed MW:
38kDa
This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. RRID AB_2758122 Gene ID 10661 Swiss Prot Synonym EKLF; EKLF/KLF1; KLF1
Purification Method:
Affinity purification
Gene ID:
10661
RRID:
AB_2758122
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat plasma, using KLF1 Rabbit pAb (CAB10581) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
