The [KO Validated] KDM1 Antibody (CAB1156) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] KDM1 Antibody
SKU:
CAB1156
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AOF2, CPRF, KDM1, LSD1, BHC110
Positive Sample:
MCF7, 293T
Cellular Localization:
Nucleus.
Calculated MW:
93kDa
Observed MW:
117kDa
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
23028
RRID
AB_2721240
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using KDM1 Rabbit pAb (CAB1156) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020).
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg KDM1 antibody (CAB1156). Western blot was performed from the immunoprecipitate using KDM1 antibody (CAB1156) at a dilution of 1:500.
