The Mouse HDGF (Hepatoma-Derived Growth Factor) CLIA Kit is specifically designed for the accurate detection of HDGF levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.HDGF is a key growth factor that plays a crucial role in promoting cell proliferation and survival. It is implicated in various biological processes, including cell growth, wound healing, and tissue regeneration.
HDGF has also been linked to cancer progression, making it a valuable biomarker for studying cancer biology and potential therapeutic targets.Overall, the Mouse HDGF CLIA Kit provides researchers with a powerful tool for investigating the biological functions of HDGF and its potential implications in various disease states.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HDGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HDGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HDGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HDGF. You can calculate the concentration of Mouse HDGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
91-104
98-110
Average (%)
93
96
105
1:4
Range (%)
87-100
92-108
100-116
Average (%)
94
99
106
1:8
Range (%)
90-103
102-117
102-119
Average (%)
96
109
109
1:16
Range (%)
100-114
92-106
101-118
Average (%)
107
99
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
100-115
106
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.22
79.23
177.84
22.06
72.82
163.55
Standard deviation
2.92
6.28
13.62
2.47
6.82
14.64
C V (%)
12.58
7.93
7.66
11.2
9.37
8.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HDGF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HDGF in samples. No significant cross-reactivity or interference between Mouse HDGF and analogues was observed.
