The ONECUT2 Polyclonal Antibody (PAC049834) is a valuable tool for researchers studying the transcription factor ONECUT2, which plays a key role in regulating gene expression and differentiation in various cell types. This antibody, produced in rabbits, exhibits strong reactivity with both human and mouse samples, making it versatile for a wide range of experimental applications.ONECUT2 is known for its involvement in developmental processes, particularly in the liver and pancreas, where it influences cell fate determination and organogenesis. Its dysregulation has been linked to various diseases, including liver cancer and diabetes, making it a promising target for therapeutic interventions.
With its specificity and sensitivity, the ONECUT2 Polyclonal Antibody enables precise detection and analysis of ONECUT2 protein levels in different biological samples, facilitating research into the molecular mechanisms underlying various diseases and potentially leading to the development of novel treatment strategies. This antibody is validated for use in techniques such as Western blotting, immunofluorescence, and immunohistochemistry, making it a valuable tool for researchers in the fields of molecular biology, cell biology, and medicine.
Western Blot. Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate. All lanes: ONECUT2 antibody at 2.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55 kDa. Observed band size: 55 kDa..
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO49834 at dilution of 1:100.
Background:
Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B.
Synonyms:
One cut domain family member 2 (Hepatocyte nuclear factor 6-beta) (HNF-6-beta) (One cut homeobox 2) (Transcription factor ONECUT-2) (OC-2), ONECUT2, HNF6B
UniProt Protein Function:
ONECUT2: Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B. Belongs to the CUT homeobox family.Protein type: Cell development/differentiation; DNA-binding; Transcription factor; Motility/polarity/chemotaxisChromosomal Location of Human Ortholog: 18q21.31Cellular Component: actin cytoskeleton; nucleoplasm; nucleusBiological Process: cell differentiation; organ morphogenesis; positive regulation of transcription from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the onecut family of transcription factors, which are characterized by a cut domain and an atypical homeodomain. The protein binds to specific DNA sequences and stimulates expression of target genes, including genes involved in melanocyte and hepatocyte differentiation. [provided by RefSeq, Jul 2008]
