OTC(Oxytetracycline) ELISA Kit (FSES0080)

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Technical Manual

OTC(Oxytetracycline) ELISA Kit (FSES0080)

Kit Name: OTC(Oxytetracycline) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Oxytetracycline (OTC) in samples, such as meat, raw milk, feed, etc.
Sensitivity: 0.3 ppb (ng/mL)
Reaction Mode: 25°C, 30 min˜15 min
Detection Limit: Meat, Milk, Egg---6 ppb; Feed---400ppb
Cross Reactivity: Oxytetracycline ---100%, Chlortetracycline --- 300%, Tetracycline---206%, Doxycycline --- 18.5%
Sample Recovery Rate: 90%±30%.
Kits Components:
Item Specifications
ELISA Microplate 96 wells
Standard Liquid 1.5 mL each (0 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb, 24.3 ppb)
Antibody Working Solution 7 mL
HRP Conjugate 7 mL
20×Concentrated Sample Diluent 50 mL
Substrate Reagent A 6 mL
Substrate Reagent B 6 mL
Stop Solution 6 mL
20×Concentrated Wash Buffer 25 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Other materials required but not supplied:
Item Specifications
Instruments: Microplate Reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01 g).
Micropipettor: Single-channel (20-200 µL, 100-1000 µL), Multi-channel (300 µL).
Reagents: Trichloroacetic acid, Na2 Fe (CN)5 NO 2H2O, ZnSO4 7H2O.
Storage And Expiry Date: Store the kit at 2-8°C. Avoid freeze. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2-8°C.