The PAI-1/Serpin E1 Monoclonal Antibody (CAB19096) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. The protein also functions as a component of innate antiviral immunity. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. RRID AB_2862588 Gene ID 5054 Swiss Prot Synonym PAI; PAI1; PAI-1; PLANH1; PAI-1/Serpin E1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Monkey samples.
Product Name:
PAI-1/Serpin E1 Monoclonal Antibody
SKU:
CAB19096
Size:
100μL, 20μL
Reactivity:
Human, Monkey
Clone Number:
ARC0473
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PAI, PAI1, PAI-1, PLANH1, PAI-1/Serpin E1
Positive Sample:
HuH-7, U-2 OS, COS-7
Cellular Localization:
Secreted.
Calculated MW:
45kDa
Observed MW:
45kDa
This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. The protein also functions as a component of innate antiviral immunity. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. RRID AB_2862588 Gene ID 5054 Swiss Prot Synonym PAI; PAI1; PAI-1; PLANH1; PAI-1/Serpin E1
Purification Method:
Affinity purification
Gene ID:
5054
RRID:
AB_2862588
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PAI-1/Serpin E1 Rabbit mAb (CAB19096) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
