The PFN2 Polyclonal Antibody (CAB21539) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. RRID Gene ID 5217 Swiss Prot Synonym PFL; D3S1319E; PFN2
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PFN2 Polyclonal Antibody
SKU:
CAB21539
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PFL, D3S1319E, PFN2
Positive Sample:
U-251 MG, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
15kDa
Observed MW:
15kDa
The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. RRID Gene ID 5217 Swiss Prot Synonym PFL; D3S1319E; PFN2
Purification Method:
Affinity purification
Gene ID:
5217
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PFN2 Rabbit pAb (CAB21539) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
