The Phospho-CREB1-S129 Antibody (CABP0903) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. RRID AB_2771005 Gene ID 1385 Swiss Prot Synonym CREB; CREB-1; Phospho-CREB1-S129
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-CREB1-S129 Antibody
SKU:
CABP0903
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CREB, CREB-1, Phospho-CREB1-S129
Positive Sample:
293T treated with UV, C6 treated with UV
Cellular Localization:
Nucleus.
Calculated MW:
35kDa
Observed MW:
43kDa
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. RRID AB_2771005 Gene ID 1385 Swiss Prot Synonym CREB; CREB-1; Phospho-CREB1-S129
Purification Method:
Affinity purification
Gene ID:
1385
RRID:
AB_2771005
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-CREB1-S129 Rabbit pAb (CABP0903) at 1:500 dilution. 293T cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from C6 cells, using Phospho-CREB1-S129 Rabbit pAb (CABP0903) at 1:500 dilution. C6 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
