Recombinant Human CAMK1D Protein (GST Tag) (RPES3985)

SKU:
RPES3985
€361

Description

ELISA Kit Technical Manual

Recombinant Human CAMK1D Protein (GST Tag)

Background:CAMK1D is a serine threonine kinase that is a member of the calcium?calmodulin-dependent protein kinase family. It contains a protein kinase domain. CAMK1D expressed in polymorphonuclear leukocytes and may be part of the chemokine signal transduction pathway that regulates granulocyte function. It may also be involved in modulation of neuronal apoptosis. It activates CREB-dependent gene transcription, regulates calcium-mediated granulocyte function and respiratory burst and promotes basal dendritic growth of hippocampal neurons.
Name:Recombinant Human CAMK1D Protein (GST Tag)
Size:10µg
Assay Genie SKU:RPES3985
Synonyms:Calcium/calmodulin-dependent protein kinase type 1D; CaM kinase I delta; CaMKI-like protein kinase; CAMK1D; CaMK1 delta;CaM-K1;CKLiK
Species:Human
Expression host:E.coli
Sequence:Met1-Lys385
Accession:Q8IU85
Mol Mass:69.2 kDa
AP Mol Mass:60-70 kDa
Tag:N-GST
Purity:> 90% as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice.Upon receipt, store it immediately at<-20°C.
Formulation:Supplied as a 0.2 um filtered solution of 20mM PB?150mM NaCl,pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:CAMK1D: a calcium/calmodulin-dependent S/T protein kinase of the CAMK group. Binding of calmodulin is thought to result in a conformational change and can lead to subsequent activation through phosphorylation by CAMKK1 and CAMKK2. Can be activated by CAMKK1 in a calcium-independent manner. Its Ca(2+)/CaM-dependent activity is enhanced 30-fold in vitro by phosphorylation at Thr180 by CaMKK1. Broadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1. Expression increases upon treatment of EC cells with DMSO and retinoic acid. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: cytoplasm; nucleus

Molecular Function:calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: positive regulation of apoptosis; positive regulation of phagocytosis; activation of CREB transcription factor; regulation of dendrite development; inflammatory response; protein amino acid phosphorylation; negative regulation of apoptosis

NCBI Summary:This gene is a member of the calcium/calmodulin-dependent protein kinase 1 family, a subfamily of the serine/threonine kinases. The encoded protein is a component of the calcium-regulated calmodulin-dependent protein kinase cascade. It has been associated with multiple processes including regulation of granulocyte function, activation of CREB-dependent gene transcription, aldosterone synthesis, differentiation and activation of neutrophil cells, and apoptosis of erythroleukemia cells. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jan 2015]
UniProt Code:Q8IU85
NCBI GenInfo Identifier:56404610
NCBI Gene ID:57118
NCBI Accession:Q8IU85.1
UniProt Related Accession:Q8IU85
Molecular Weight:45kDa
NCBI Full Name:Calcium/calmodulin-dependent protein kinase type 1D
NCBI Synonym Full Names:calcium/calmodulin dependent protein kinase ID
NCBI Official Symbol:CAMK1D  
NCBI Official Synonym Symbols:CKLiK; CaM-K1; CaMKID  
NCBI Protein Information:calcium/calmodulin-dependent protein kinase type 1D
UniProt Protein Name:Calcium/calmodulin-dependent protein kinase type 1D
UniProt Synonym Protein Names:CaM kinase I delta; CaM kinase ID; CaM-KI delta; CaMKI delta; CaMKID; CaMKI-like protein kinase; CKLiK
Protein Family:Calcium/calmodulin-dependent protein kinase
UniProt Gene Name:CAMK1D  
UniProt Entry Name:KCC1D_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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