Recombinant Human CD16b/FCGR3B Protein (Active) (RPES4127)



ELISA Kit Technical Manual

Recombinant Human CD16b/FCGR3B Protein (Active)

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Additional Information

Background:Carbohydrate sulfotransferase 15, also known as N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase, GalNAc4S-6ST, B-cell RAG-associated gene protein, CHST15 and BRAG, is a single-pass type I I membrane protein which belongs to the sulfotransferase 1 family. CHST15 / BRAG is expressed in B-cell-enriched tissues but not in fetal or adult thymus. It is expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. It is not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. CHST15 / BRAG is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. CHST15 / BRAG may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and / or regulation of B-cell-specific RAG expression.
Name:Recombinant Human CD16b/FCGR3B Protein (Active)
Assay Genie SKU:RPES4127
Synonyms:Low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma RIII-beta; FcR0; IgG Fc receptor III; FCG3; FCGR3; CD16b and FCGR4B;FCRIII;FCRIIIb
Expression host:E.coli
Sequence:Met 18-Gly 193
Mol Mass:20 kDa
AP Mol Mass:22 kDa
Bio Activity:1.Using the Octet RED System, the affinity constant (Kd) of CD16b bound to Human IgG1 was 80nM.
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:Please contact us for more information.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 7.4
Reconstitution:Please refer to the printed manual for detailed information.
Product type:
Recombinant Protein
Host species:
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