Recombinant Human HVEM/TNFRSF14 Protein (mFc Tag) (RPES2259)



ELISA Kit Technical Manual

Recombinant Human HVEM/TNFRSF14 Protein (mFc Tag)

Background:Herpesvirus entry mediator (HVEM) is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity. HVEM is highly expressed on na?ve CD4+ T cells, CD8+ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils. It functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha. Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation and contributes to Th1 inflammation and cardiac allograft rejection. In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation and dampens intestinal inflammation. HVEM enhances the development of CD8+ T cell memory and Treg function. It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense. The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding.
Name:Recombinant Human HVEM/TNFRSF14 Protein (mFc Tag)
Assay Genie SKU:RPES2259
Synonyms:Tumor Necrosis Factor Receptor Superfamily Member 14; Herpes Virus Entry Mediator A; Herpesvirus Entry Mediator A; HveA; Tumor Necrosis Factor Receptor-Like 2; TR2; CD270; TNFRSF14; HVEA; HVEM
Expression host:Human Cells
Mol Mass:44.1 kDa
AP Mol Mass:55-90 kDa
Purity:> 95% as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Lyophilized protein should be stored at < -20'C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of PBS, 150mM NaCl, pH 7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:TNFRSF14: Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
UniProt Protein Details:

Protein type:Receptor, misc.; Membrane protein, integral; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p36.32

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function:viral receptor activity; protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; entry of virus into host cell; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; T cell costimulation; positive regulation of cytokine secretion during immune response; negative regulation of alpha-beta T cell proliferation; immune response; defense response to Gram-negative bacterium

NCBI Summary:This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Code:Q92956
NCBI GenInfo Identifier:13878821
NCBI Gene ID:8764
NCBI Accession:Q92956.3
UniProt Secondary Accession:Q92956,Q6IB95, Q8N634, Q8WXR1, Q96J31, Q9UM65, B3KW30 B9DI89,
UniProt Related Accession:Q92956
Molecular Weight:283
NCBI Full Name:Tumor necrosis factor receptor superfamily member 14
NCBI Synonym Full Names:tumor necrosis factor receptor superfamily, member 14
NCBI Official Symbol:TNFRSF14
NCBI Official Synonym Symbols:TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
NCBI Protein Information:tumor necrosis factor receptor superfamily member 14; CD40-like protein; herpes virus entry mediator A; tumor necrosis factor receptor-like gene2; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
UniProt Protein Name:Tumor necrosis factor receptor superfamily member 14
UniProt Synonym Protein Names:Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; Tumor necrosis factor receptor-like 2; TR2; CD_antigen: CD270
Protein Family:Tumor necrosis factor receptor superfamily
UniProt Gene Name:TNFRSF14
UniProt Entry Name:TNR14_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
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