Recombinant Human SerpinF1/PEDF Protein (His Tag) (RPES4718)



ELISA Kit Technical Manual

Recombinant Human SerpinF1/PEDF Protein (His Tag)

Background:Serpin F1 is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins. Serpin F1 is a new promising approach for the treatment of osteosarcoma and has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Levels of the natural ocular anti-angiogenic factor SentrinF1 (PEDF) is associated with proliferative retinopathy.
Name:Recombinant Human SerpinF1/PEDF Protein (His Tag)
Assay Genie SKU:RPES4718
Synonyms:Pigment Epithelium-Derived Factor; PEDF; Cell Proliferation-Inducing Gene 35 Protein; EPC; Serpin F1; SERPINF1; PEDF;OI12;OI6;PEDF;PIG35
Expression host:Human Cells
Mol Mass:45.4 kDa
AP Mol Mass:50 kDa
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 4421) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: axon hillock; extracellular matrix; extracellular space; perinuclear region of cytoplasm; extracellular region; melanosome; basement membrane

Molecular Function:serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; positive regulation of neurogenesis; negative regulation of angiogenesis; ovulation cycle; retina development in camera-type eye; short-term memory; response to arsenic; multicellular organismal development; negative regulation of inflammatory response; response to acidity; kidney development; aging

Disease: Osteogenesis Imperfecta, Type Vi

NCBI Summary:The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]
UniProt Code:P36955
NCBI GenInfo Identifier:313104314
NCBI Gene ID:5176
NCBI Accession:P36955.4
UniProt Secondary Accession:P36955,Q13236, Q2TU83, Q96CT1, Q96R01, Q9BWA4, F1T092
UniProt Related Accession:P36955
Molecular Weight:46,312 Da
NCBI Full Name:Pigment epithelium-derived factor
NCBI Synonym Full Names:serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
NCBI Official Symbol:SERPINF1
NCBI Official Synonym Symbols:OI6; OI12; PEDF; EPC; PIG35
NCBI Protein Information:pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
UniProt Protein Name:Pigment epithelium-derived factor
UniProt Synonym Protein Names:Cell proliferation-inducing gene 35 protein; EPC; Serpin F1
UniProt Gene Name:SERPINF1
UniProt Entry Name:PEDF_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
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