Recombinant Human SerpinH1/SERPINH1 Protein (His Tag) (RPES4763)

SKU:
RPES4763
€196

Description

ELISA Kit Technical Manual

Recombinant Human SerpinH1/SERPINH1 Protein (His Tag)

Background:Serpin H1 is a serine proteinase inhibitors Which belongs to the serpin family. Serpin H1 is induced by heat shock. Serpin H1 localizes to the endoplasmic reticulum lumen and binds specifically to collagen. Thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. Serpin H1 may be a marker for cancer and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes.
Name:Recombinant Human SerpinH1/SERPINH1 Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES4763
Synonyms:Serpin H1; 47 kDa Heat Shock Protein; Arsenic-Transactivated Protein 3; AsTP3; Cell Proliferation-Inducing Gene 14 Protein; Collagen-Binding Protein; Colligin; Rheumatoid Arthritis-Related Antigen RA-A47; SERPINH1; CBP1; CBP2; HSP47; SERPINH2
Species:Human
Expression host:Human Cells
Sequence:Ala19-Leu418
Accession:P50454
Mol Mass:45.5 kDa
AP Mol Mass:51 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:SERPINH1: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10). A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae. Belongs to the serpin family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: extracellular space; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment

Molecular Function:serine-type endopeptidase inhibitor activity; collagen binding; unfolded protein binding

Biological Process: extracellular matrix organization and biogenesis; collagen fibril organization; protein maturation; collagen biosynthetic process; response to unfolded protein

Disease: Osteogenesis Imperfecta, Type X; Preterm Premature Rupture Of The Membranes

NCBI Summary:This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]
UniProt Code:P50454
NCBI GenInfo Identifier:20141241
NCBI Gene ID:871
NCBI Accession:P50454.2
UniProt Secondary Accession:P50454,P29043, Q5XPB4, Q6NSJ6, Q8IY96, Q9NP88, B3KVJ3
UniProt Related Accession:P50454
Molecular Weight:418
NCBI Full Name:Serpin H1
NCBI Synonym Full Names:serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
NCBI Official Symbol:SERPINH1
NCBI Official Synonym Symbols:CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
NCBI Protein Information:serpin H1; colligin; colligin-2; 47 kDa heat shock protein; arsenic-transactivated protein 3; rheumatoid arthritis antigen A-47; cell proliferation-inducing gene 14 protein; rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase in
UniProt Protein Name:Serpin H1
UniProt Synonym Protein Names:47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP3; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; Colligin; Rheumatoid arthritis-related antigen RA-A47
UniProt Gene Name:SERPINH1
UniProt Entry Name:SERPH_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
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