Description
Recombinant Human UBE2G1 Protein
Background: | UBE2G1 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. |
Name: | Recombinant Human UBE2G1 Protein |
Size: | 50µg |
Assay Genie SKU: | RPES0816 |
Synonyms: | E217K;UBC7;UBE2G |
Species: | Human |
Expression host: | E.coli |
Sequence: | Met 1-Glu 170 |
Accession: | P62253 |
Mol Mass: | 19.5 kDa |
AP Mol Mass: | 19.5 kDa |
Tag: | |
Purity: | > 85 % as determined by reducing SDS-PAGE. |
Endotoxin: | Please contact us for more information. |
Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months. |
Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation: | Lyophilized from sterile PBS, 10% glycerol, pH 7.5 |
Reconstitution: | Please refer to the printed manual for detailed information. |
UniProt Protein Function: | UBE2G1: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4. Belongs to the ubiquitin-conjugating enzyme family. |
UniProt Protein Details: | Protein type:EC 6.3.2.19; Ligase; Ubiquitin ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 17p13.2 Cellular Component: cytoplasm Molecular Function:small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; cellular protein catabolic process |
NCBI Summary: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. [provided by RefSeq, Jul 2008] |
UniProt Code: | P62253 |
NCBI GenInfo Identifier: | 51338681 |
NCBI Gene ID: | 7326 |
NCBI Accession: | P62253.3 |
UniProt Secondary Accession: | P62253,Q99462, B2R7P2, D3DTK0, |
UniProt Related Accession: | P62253 |
Molecular Weight: | 170 |
NCBI Full Name: | Ubiquitin-conjugating enzyme E2 G1 |
NCBI Synonym Full Names: | ubiquitin-conjugating enzyme E2G 1 |
NCBI Official Symbol: | UBE2G1 |
NCBI Official Synonym Symbols: | UBC7; E217K; UBE2G |
NCBI Protein Information: | ubiquitin-conjugating enzyme E2 G1; ubiquitin-protein ligase G1; ubiquitin carrier protein G1; ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast); ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans); ubiquitin-conjugating enzyme E2G 1 (homologous to C. elegans UBC7) |
UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 G1 |
UniProt Synonym Protein Names: | E217K; UBC7; Ubiquitin carrier protein G1; Ubiquitin-protein ligase G1Ubiquitin-conjugating enzyme E2 G1, N-terminally processed |
Protein Family: | Ubiquitin-conjugating enzyme |
UniProt Gene Name: | UBE2G1 |
UniProt Entry Name: | UB2G1_HUMAN |
Additional Information
Product type: |
Recombinant Protein |
Host species: |
E.coli |
Reactivity: |
Human |