Recombinant Human UBE2G1 Protein (RPES0816)

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ELISA Kit Technical Manual

Recombinant Human UBE2G1 Protein

Background:UBE2G1 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. 
Name:Recombinant Human UBE2G1 Protein
Assay Genie SKU:RPES0816
Expression host:E.coli
Sequence:Met 1-Glu 170
Mol Mass:19.5 kDa
AP Mol Mass:19.5 kDa
Purity:> 85 % as determined by reducing SDS-PAGE.
Endotoxin:Please contact us for more information.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from sterile PBS, 10% glycerol, pH 7.5
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:UBE2G1: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4. Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:

Protein type:EC; Ligase; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: cytoplasm

Molecular Function:small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; cellular protein catabolic process

NCBI Summary:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. [provided by RefSeq, Jul 2008]
UniProt Code:P62253
NCBI GenInfo Identifier:51338681
NCBI Gene ID:7326
NCBI Accession:P62253.3
UniProt Secondary Accession:P62253,Q99462, B2R7P2, D3DTK0,
UniProt Related Accession:P62253
Molecular Weight:170
NCBI Full Name:Ubiquitin-conjugating enzyme E2 G1
NCBI Synonym Full Names:ubiquitin-conjugating enzyme E2G 1
NCBI Official Symbol:UBE2G1  
NCBI Official Synonym Symbols:UBC7; E217K; UBE2G  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 G1; ubiquitin-protein ligase G1; ubiquitin carrier protein G1; ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast); ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans); ubiquitin-conjugating enzyme E2G 1 (homologous to C. elegans UBC7)
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 G1
UniProt Synonym Protein Names:E217K; UBC7; Ubiquitin carrier protein G1; Ubiquitin-protein ligase G1Ubiquitin-conjugating enzyme E2 G1, N-terminally processed
Protein Family:Ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2G1  
UniProt Entry Name:UB2G1_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
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