Ubiquitin-conjugating E2 family member UBE2G1 is involved in the second step of the ubiquitination process. Ubiquitination has long been thought to be the central mechanism for protein degradation, but it is now considered important for a variety of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To handle this complexity and allow for adequate ubiquitination in time and space, a sophisticated conjugation mechanism has been created. In a stepwise manner, ubiquitin is activated by an ubiquitin-activating enzyme (E1), which delivers the ubiquitin to a set of ubiquitin-conjugating enzymes (E2s). Finally, E2s loaded with ubiquitin are interacting with ubiquitin protein ligases (E3s) and are conjugated to substrates as a result of recruitment by the E3. The three fundamental enzymes in this hierarchy are working in a hierarchical system, with two E1s and 35 E2s having been discovered and hundreds of E3s being identified in people. Human UBE2G1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human UBE2G1 Recombinant Protein (RPES0816)
Product Code:
RPES0816
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
E217K,UBC7,UBE2G
Accession:
P62253
Sequence:
Met 1-Glu 170
Fusion tag:
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human UBE2G1 (P62253) (Met 1-Glu 170) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
19.5 kDa
AP Mol Mass:
19.5 kDa
Formulation:
Lyophilized from sterile PBS, 10% glycerol, pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
