Mouse Cathepsin B/CTSB Recombinant Protein (RPES4639)

SKU:
RPES4639
Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Mouse
€196

Description

ELISA Kit Technical Manual

Recombinant Mouse Cathepsin B/CTSB Protein (His Tag)

Background:Cathepsin B (CatB) is an enzymatic protein belonging to the peptidase (or protease) families. It is the first described member of the family of lysosomal cysteine proteases. It is known to process a number of proteins, including pro and active caspases, prorenin and secretory leucoprotease inhibitor (SLPI). It is believed to participate in intracellular degradation and turnover of proteins. Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Cathepsin B may also have a role in invasion and metastasis.
Name:Recombinant Mouse Cathepsin B/CTSB Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES4639
Synonyms:Cathepsin B;Ctsb;Cathepsin B1
Species:Mouse
Expression host:Human Cells
Sequence:Met 1-Phe 339
Accession:P10605
Mol Mass:36.4 kDa
AP Mol Mass:32-50kDa
Tag:C-6His
Purity:> 95 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:LyopHilized from a 0.2 um filtered solution of PBS, pH 7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; EC 3.4.22.1; Protease; Autophagy

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; mitochondrion; lysosome; extracellular region; caveola; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; nucleolus; sarcolemma; external side of plasma membrane

Molecular Function:peptidase activity; collagen binding; proteoglycan binding; protein self-association; hydrolase activity; cysteine-type endopeptidase activity; endopeptidase activity; protein complex binding; kininogen binding; peptide binding; cysteine-type peptidase activity

Biological Process: collagen catabolic process; proteolysis involved in cellular protein catabolic process; regulation of catalytic activity; decidualization; proteolysis

NCBI Summary:This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015]
UniProt Code:P10605
NCBI GenInfo Identifier:115712
NCBI Gene ID:13030
NCBI Accession:P10605.2
UniProt Secondary Accession:P10605,Q3UDW7,
UniProt Related Accession:P10605
Molecular Weight:37,280 Da
NCBI Full Name:Cathepsin B
NCBI Synonym Full Names:cathepsin B
NCBI Official Symbol:Ctsb  
NCBI Official Synonym Symbols:CB  
NCBI Protein Information:cathepsin B; cathepsin B1
UniProt Protein Name:Cathepsin B
UniProt Synonym Protein Names:Cathepsin B1Cleaved into the following 2 chains:Cathepsin B light chain; Cathepsin B heavy chain
Protein Family:Cathepsin
UniProt Gene Name:Ctsb  
UniProt Entry Name:CATB_MOUSE