SEM (Nitrofurazone) ELISA Kit (FSES0005)

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Technical Manual

SEM (Nitrofurazone) ELISA Kit

Kit Name: SEM (Nitrofurazone) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect SEM in samples, such as honey, muscle, milk, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the reaction, SEM in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti- SEM antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of SEM. The concentration of SEM in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.05 ppb (ng/mL)
Reaction Mode: 25°C, 45 min~ 15 min
Detection Limit: Muscle,Liver, Honey, Milk, Egg---0.1 ppb, Muscle (Fish, shrimp)---0.15 ppb Milk powder, Egg powder, Feed---0.1 ppb,
Cross Reactivity: Nitrofurazone metabolite---100%, Furazolidone metabolite--- <0.1%, Aminohydantoin Hydrochloride --- <0.1%, Furaltadone metabolite--- <0.1%
Sample Recovery Rate: Muscle, Liver, Egg---90%±15%; Honey, Milk ---80%±15%; Milk powder, Egg powder, Feed --85%±25%
Kits Components:
Item Specifications
ELISA Microtiter plate 96 wells
Standard Liquid 1 mL each (0ppb,0.05ppb,0.15ppb,0.45ppb,1.35ppb,4.05ppb)
Derivatization Reagent 10 mL
HRP Conjugate 5.5 mL
Antibody Working Solution 5.5 mL
Substrate Reagent A 6 mL
Substrate Reagent B 6 mL
Stop Solution 6 mL
20 x Concentrated Wash Buffer 40 mL
2 x Reconstitution Buffer 50 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: Ethyl acetate, N-hexane, NaOH, Concentrated HCl,K2HPO4 3H2O, ZnSO4 7H2O, Na2Fe (CN) 5NO 2H2O.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.