The SMG5 Antibody (CAB18123) is a high-quality antibody developed for reliable detection and analysis of target proteins. SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al., 2003 [PubMed 14636577]).
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
SMG5 Antibody
SKU:
CAB18123
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EST1B, SMG-5, LPTSRP1, LPTS-RP1, SMG5
Positive Sample:
Mouse spleen, Mouse brain
Cellular Localization:
Cytoplasm, Cytosol, Nucleus.
Calculated MW:
114kDa
Observed MW:
114kDa
SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al., 2003 [PubMed 14636577]).
Purification Method
Affinity purification
Gene ID
23381
RRID
AB_2861914
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SMG5 Rabbit pAb (CAB18123) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
